Eur. J. Entomol. 105 (4): 567-574, 2008 | 10.14411/eje.2008.076

Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution

Zhong Zheng GUI1,2, Bo Yeon KIM1, Kwang Sik LEE1, Ya Dong WEI1,2, Xijie GUO2, Hung Dae SOHN1, Byung Rae JIN*,1
1 College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea
2 Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China

Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.

Keywords: Baculovirus expression vector, Bombyx mori, cDNA cloning, enzyme, gene structure, glutathione S-transferase, silkworm

Received: January 15, 2008; Accepted: February 18, 2008; Published: October 24, 2008

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