Eur. J. Entomol. 105 (4): 561-566, 2008 | 10.14411/eje.2008.075

Leucine-rich fibroin gene of the Japanese wild silkmoth, Rhodinia fugax (Lepidoptera: Saturniidae)

Hideki SEZUTSU, Toshiki TAMURA, Kenji YUKUHIRO*
National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan; e-mail: kygnis@affrc.go.jp

We cloned and characterized a partial fibroin gene of Rhodinia fugax (Saturniidae). The gene encodes a fibroin consisting mainly of orderly arranged repeats, each of which is divided into a polyalanine and a nonpolyalanine block, similar to the fibroins of Antheraea pernyi and A. yamamai. Three repeat types differ in the sequence of the nonpolyalanine block. In contrast to the Antheraea fibroins, the fibroin of R. fugax is rich in glutamate and leucine residues (about 3% and 5%, respectively) and contains less alanine.

Keywords: Fibroin, Rhodinia fugax, repeat, polyalanine

Received: May 21, 2008; Accepted: July 9, 2008; Published: October 24, 2008

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References

  1. DATTA A., GHOSH A.K. & KUNDU S.C. 2001: Differential expression of the fibroin gene in developmental stages of silkworm, NSaturniidae. Comp. Biochem. Physiol. (B) 129: 197-204 Go to original source...
  2. GOSLINE J.M., GUERETTE P.A., ORTLEPP C.S. & SAVAGE K.N. 1999: The mechanical design of spider silks: from fibroin sequence to mechanical function. J. Exp. Biol. 202: 3295-3303
  3. HINMAN M.B. & LEWIS R.V. 1992: Isolation of a clone encoding a second dragline silk fibroin. J. Biol. Chem. 267: 19320-19324
  4. HWANG J.S., LEE J.S., GOO T.W., YUN E.Y., LEE K.S., KIM Y.S., JIN B.R., LEE S.M., KIM K.Y., KANG S.W. & SUH D.S. 2001: Cloning of the fibroin gene from the oak silkworm, Antheraea yamamai and its complete sequence. Biotechnol. Lett. 23: 1321-1326 Go to original source...
  5. INOUE H., SUGI S., KUROKO H., MORIUCHI S., KAWABE J. & OWADA M. 1982: Moths of Japan. Kodansha, Tokyo, 589 pp
  6. INOUE S., TANAKA K., ARISAKA F., KIMURA S., OHTOMO K. & MIZUNO S. 2000: Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6 : 6 : 1 molar ratio. J. Biol. Chem. 275: 40517-40528 Go to original source...
  7. JEFFREYS A.J., WILSON V. & THEIN S.L. 1985: Hypervariable "minisatellite" regions in human DNA. Nature 315: 67-73 Go to original source...
  8. KIRIMURA J. 1962: Studies on amino acid composition and chemical structure of silk protein by microbiological determination. Bull. Sericul. Exp. Stat . 17: 447-522 [in Japanese]
  9. SAMBROOK J., FRITSCH F.E. & MANIATIS T. 1989: Molecular Cloning. Cold Spring Harbor Laboratory Press, New York, 659 pp
  10. SEZUTSU H. & YUKUHIRO K. 2000: Dynamic rearrangement within the Antheraea pernyi silk fibroin gene is associated with four types of repetitive units. J. Mol. Evol. 51: 329-338 Go to original source...
  11. TAKASU Y., YAMADA H., TAMURA T., SEZUTSU H., MITA K. & TSUBOUCHI K. 2007: Identification and characterization of a novel sericin gene expressed in the anterior middle silk gland of the silkworm Bombyx mori. Insect Biochem Mol. Biol. 37: 1234-1240 Go to original source...
  12. TAMURA T., INOUE H. & SUZUKI Y. 1987: The fibroin genes of the Antheraea yamamai and Bombyx mori are different in the core regions but reveal a striking sequences similarity in their 58-ends and 58-flanking regions. Mol. Gen. Genet. 206: 189-195 Go to original source...
  13. TAMURA T., THIBERT C., ROYER C., KANDA T., ABRAHAM E., KAMBA M., KOMOTO N., THOMAS J.L., MAUCHAMP B., CHAVANCY G., SHIRK P., FRASER M., PRUDHOMME J.C. & COUBLE P. 2000: Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat. Biotechnol. 18: 81-84 Go to original source...
  14. TANAKA K. & MIZUNO S. 2001: Homologues of fibroin L-chain and P25 of Bombyx mori are present in Dendrolimus spectabilis and Papilio xuthus but not detectable in Antheraea yamamai. Insect Biochem. Mol. Biol. 31: 665-677 Go to original source...
  15. XU M. & LEWIS R.V. 1990: Structure of a protein superfiber: spider dragline silk. Proc. Natl. Acad. Sci. USA 87: 7120-7124 Go to original source...
  16. YAGO M. & MITAMURA T. 1999: Specific structure in the pupal cuticle of the Japanese wild silkmoth, Rhodinia fugax Butler. Int. J. Wild Silkmoth Silk 4: 39-42
  17. ZHOU C.Z., CONFALONIERI F., MEDINA N., ZIVANOVIC Y., ESNAULT C., YANG T., JACQUET M., JANIN J., DUGUET M., PERASSO R. & LI Z.G. 2000: Fine organization of Bombyx mori fibroin heavy chain gene. Nucl. Acids Res. 28: 2413-2419 Go to original source...