Eur. J. Entomol. 105 (2): 361-362, 2008 | DOI: 10.14411/eje.2008.043

Polymorphic microsatellite loci in the endangered butterfly Lycaena helle (Lepidoptera: Lycaenidae)

Jan Christian HABEL1, Aline FINGER2, Marc MEYER1, Thomas SCHMITT2, Thorsten ASSMANN3
1 Musée National d'Histoire Naturelle, Section Zoologie des Invertébrés, 25, rue Münster, L-2160 Luxembourg; e-mail: Janchristianhabel@gmx.de
2 Biogeographie, Universität Trier, D-54296 Trier, Germany
3 Leuphana Universität Lüneburg, Institut für Ökologie und Umweltchemie, D-21335 Lüneburg, Germany

Six polymorphic microsatellite loci were isolated in the endangered butterfly Lycaena helle. Five of them provided interpretable results. We detected four to 34 alleles per locus in a total of 235 samples (males and females) collected from meadows in the Ardennes-Eifel (Germany, Luxemburg and Belgium) and the Westerwald (Germany). We collected one leg for DNA-extraction as a non-lethal method. The expected heterozygosities ranged from 48.6% to 83.1%, depending on the locus analysed. These markers are currently being used in our studies of the species´ phylogeography over its western Palearctic distribution area and for the analysis of the conservation status of the fragmented populations in Central Europe.

Keywords: Lepidoptera, Lycaenidae, Lycaena helle, microsatellite marker

Received: June 15, 2007; Accepted: January 21, 2008; Revised: January 21, 2008; Published: May 15, 2008Show citation

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HABEL, J.C., FINGER, A., MEYER, M., SCHMITT, T., & ASSMANN, T. (2008). Polymorphic microsatellite loci in the endangered butterfly Lycaena helle (Lepidoptera: Lycaenidae). Eur. J. Entomol.105(2), 361-362. doi: 10.14411/eje.2008.043.
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References

  1. EBERT G. & RENNWALD E. (ed.) 1991: Die Schmetterlinge Baden-Wuerttembergs. Vol. 1. Eugen Ulmer, Stuttgart, 552 pp
  2. FISCHER K., BEINLICH B. & PLACHTER H. 1999: Population structure, mobility and habitat preferences of the violet copper Lycaena helle (Lepidoptera: Lycaenidae) in Western Germany: implications for conservation. J. Conserv. 3: 43-52 Go to original source...
  3. GRUENSTEIN M. & HOGNESS D.S. 1975: Colony hybridization. Method for the isolation of cloned DNA's that contain a specific gene. Proc. Natl. Acad. Sci. 72: 3961-3965 Go to original source...
  4. HAMILTON M.B., PINCUS E.L., DI FIORE A. & FLEISCHER R.C. 1999: Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques. 27: 500-507
  5. IUCN 1996: The 1996 IUCN Red List of Threatened Animals. IUCN Publications Service Unit, Cambridge, 341 pp
  6. KUDRNA O. 2002: The distribution atlas of European butterflies. Oedippus 20: 1-342
  7. LUIKART G., SHERWIN W.B., STEELE B.M. & ALLENDORF F.W. 1998: Usefulness of molecular markers for detecting population bottlenecks via monitoring genetic change. Mol. Ecol. 7: 963-974 Go to original source...
  8. OOSTERHOUT C. VAN, HUTCHINSON W.F., WILLS D.P.M., SHIPLEY P. 2004: Micro-checker: Software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Not. 4: 535-538 Go to original source...
  9. PEAKALL R. & SMOUSE P.E. 2006: GENEALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6: 288-295 Go to original source...
  10. VAN SWAAY C.A.M. & WARREN M. 1999: Red Data Book of European Butterflies (Rhopalocera). Nature and Environment 99, Council of Europe Publishing, Strasbourg, 264 pp
  11. TOLMAN T. & LEWINGTON R. 1997: Field Guide Butterflies of Britain and Europe. Harper Collins, London, 384 pp

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