Eur. J. Entomol. 105 (2): 361-362, 2008 | 10.14411/eje.2008.043

Polymorphic microsatellite loci in the endangered butterfly Lycaena helle (Lepidoptera: Lycaenidae)

Jan Christian HABEL1, Aline FINGER2, Marc MEYER1, Thomas SCHMITT2, Thorsten ASSMANN3
1 Musée National d'Histoire Naturelle, Section Zoologie des Invertébrés, 25, rue Münster, L-2160 Luxembourg; e-mail: Janchristianhabel@gmx.de
2 Biogeographie, Universität Trier, D-54296 Trier, Germany
3 Leuphana Universität Lüneburg, Institut für Ökologie und Umweltchemie, D-21335 Lüneburg, Germany

Six polymorphic microsatellite loci were isolated in the endangered butterfly Lycaena helle. Five of them provided interpretable results. We detected four to 34 alleles per locus in a total of 235 samples (males and females) collected from meadows in the Ardennes-Eifel (Germany, Luxemburg and Belgium) and the Westerwald (Germany). We collected one leg for DNA-extraction as a non-lethal method. The expected heterozygosities ranged from 48.6% to 83.1%, depending on the locus analysed. These markers are currently being used in our studies of the species´ phylogeography over its western Palearctic distribution area and for the analysis of the conservation status of the fragmented populations in Central Europe.

Keywords: Lepidoptera, Lycaenidae, Lycaena helle, microsatellite marker

Received: June 15, 2007; Accepted: January 21, 2008; Published: May 15, 2008

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