Eur. J. Entomol. 105 (2): 355-359, 2008 | 10.14411/eje.2008.042

Isolation of antibacterial response genes from the dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) immunized with Escherichia coli

Jae-Sam HWANG1, Yeon-Ju KIM1, Hea-Son BANG1, Eun-Young YUN1, Young-Tae KIM2, Seong-Ryul KIM1, Kwan-Ho PARK1, Nam-Soon KIM3, Iksoo KIM4, Seok-Jo HWANG1, Jae-Pil JEON5
1 National Institute of Agricultural Science and Technology, RDA, Suwon 441-100, South Korea; e-mail: hwangjs@rda.go.kr
2 National Institute of Agricultural Biotechnology RDA, Suwon 441-100, South Korea
3 Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, South Korea
4 College of Agriculture & Life science, Chonnam National University, Gwangju 500-757, South Korea
5 Korea National Institute of Health, Seoul 136-713, South Korea

Dot blot hybridization of total RNA from normal and bacteria-injected larvae of the dung beetle Copris tripartitus identified 13 cDNA clones that seem to be associated with the immune response. A cDNA encoding a dung beetle homolog of the heat shock protein HSP70 (CTHSP70) was characterized by full-length sequencing and expression pattern analysis. CTHSP70 was upregulated 4 h after bacterial injection, reached maximum level after 8 h, and declined gradually after 16 h. Our data suggest that CTHSP70 and another 12 genes are involved in the anti-microbial defense that is particularly important for dung beetle larvae living in pathogen-rich conditions.

Keywords: Antibacterial gene, dung beetle, HSP70

Received: September 3, 2007; Accepted: December 12, 2007; Published: May 15, 2008

Download citation

References

  1. ABE T., KONISHI T., HIRANO T., DASAI H., SHIMIZU K. & KASHIMURA M. 1995: Possible correlation between DNA damage induced by hydrogen peroxide and translocation of heat shock 70 protein into the nucleus. Biochem. Biophys. Res. Commun. 206: 548-555 Go to original source...
  2. BISCHOFF V., VIGNAL C., DUVIC B., BONECA I.G., HOFFMANN J.A. & ROYET J. 2006: Downregulation of the Drosophila immune response by peptidoglycan-recognition proteins SC1 and SC2. PLoS Pathogens 2: 139-147 Go to original source...
  3. BROWN D.C., BRADLEY B.P. & PAYNTER K.T. 1993: The physiological effects of protozoan parasitism on the eastern oyster, Crassostrea virginica: induction of stress proteins. J. Shellfish Res. 12: 135-136
  4. BULET P., HETRU C., DIMARCQ J.L. & HOFFMANN D. 1999: Antimicrobial peptides in insects; structure and function. Dev. Comp. Immunol. 23: 329-344 Go to original source...
  5. COLLINS A.M., WILLIAMS V. & EVANS J.D. 2004: Sperm storage and antioxidative enzyme expression in the honey bee, Apis mellifera. Insect Mol. Biol. 13: 141-146 Go to original source...
  6. DIMARCQ J.L., HOFFMANN D., MEISTER M., BULET P., LANOT R., REICHHART J.M. & HOFFMANN J.A. 1994: Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. Eur. J. Biochem. 221: 201-209 Go to original source...
  7. ENCOMIO V.G. & CHU F.L. 2004: Characterization of heat shock protein expression and induced thermotolerance in P. marinus parasitized eastern oysters: lab and field studies. J. Shellfish Res. 23: 289-290
  8. ENGSTROM Y. 1999: Induction and regulation of antimicrobial peptides in Drosophila. Dev. Comp. Immunol. 23: 345-358 Go to original source...
  9. FORSYTH R.B., CANDIDO E.P.M., BABICH S.L. & IWAMA G.K. 1997: Stress protein expression in coho salmon with bacterial kidney disease. J. Aquat. Anim. Health 9: 18-25 Go to original source...
  10. FUKAMACHI Y., KARASAKI Y., SUGIURA T., ITOH H., ABE T. & YAMAMURA K. 1998: Zinc suppresses apoptosis of U937 cells induced by hydrogen peroxide through an increase of the Bcl2/Bax ratio. Biochem. Biophys. Res. Commun. 246: 364-369 Go to original source...
  11. GARCIA-OROZCO K.D., LOPEZ-ZAVALA A.A., PUENTES-CAMACHO D., CALDERON-DE-LA-BARCA A.M. & SOTELO-MUNDO R.R. 2005: Recombinant bacterial expression of the lysozyme from the tobacco-hornworm Manduca sexta with activity at low temperatures. Biotechnol. Lett. 15: 1075-1080 Go to original source...
  12. GETHING M. & SAMBROOK J. 1992: Protein folding in the cell. Nature 355: 33-45 Go to original source...
  13. GOODMAN R. & BLANK M. 1998: Magnetic field stress induces expression of hsp70. Cell Stress Chaper. 3: 79-88 Go to original source...
  14. HATAYAMA T., ASAI Y., WAKATSUKI T., KITAMURA T. & IMAHARA H. 1993: Regulation of hsp70 synthesis induced by cupric sulfate and zinc sulfate in thermotolerant HeLa cells. J. Biol. Chem. 114: 592-597
  15. HARTL F.U. 1996: Molecular chaperones in cellular protein folding. Nature 381: 571-579 Go to original source...
  16. HOFFMANN J.A. 1995: Innate immunity. Curr. Opin. Immunol. 7: 4-10 Go to original source...
  17. HOFFMANN J.A. 2003: The immune response of Drosophila. Nature 426: 33-38 Go to original source...
  18. HOFFMAN J.A., KAFATOS F.C., JANEWAY C.A., EZEKOWITZ R.A. 1999: Phylogenic perspectives in innate immunity. Science 284: 1313-1318 Go to original source...
  19. HULTMARK D. 2003: Drosophila immunity: paths and patterns. Curr. Opin. Immunol. 15: 12-19 Go to original source...
  20. IWAMA GK., THOMAS P.T., FORSYTH R.B. & VIJAYAN M.M. 1998: Heat shock protein expression in fish. Rev. Fish Biol. Fish 8: 35-56 Go to original source...
  21. LEMAITRE B. 2004: The road to toll. Nat. Rev. Immunol. 4: 521-527 Go to original source...
  22. LINDQUIST S. & CRAIG E.A. 1988: The heat-shock proteins. Annu. Rev. Genet. 22: 631-677 Go to original source...
  23. MARUYAMA K. & SUGANO S. 1994: Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 138: 171-174 Go to original source...
  24. MATSUYAMA K. & NATORI S. 1988: Molecular cloning of cDNA for Sapecin and unique expression of the sapecin gene during the development of Sarcophaga peregrina. J. Biol. Chem. 263: 17117-17121
  25. MORIMOTO R.I., TISSIERES A. & GEORGOPOULOS C. 1990: The stress response, function of proteins, and oersoectives In Morimoto R.I., Tissieres A. & Georgopoulos D. (eds): Stress Proteins in Biology and Medicine. Cold Spring Harbor Laboratory, New York, pp. 1-36
  26. MORIMOTO R.I., SARGE K.D. & ABRAVAYA K. 1992: Transcriptional regulation of heat shock genes. A paradigm for inducible genomic responses. J. Biol. Chem. 267: 21987-21990
  27. MOSELEY P. 2000: Stress proteins and the immune response. Immunopharmacology 48: 299-302 Go to original source...
  28. SIERRA-RIVER E., VOORHEES G.J. & FREEMAN M.L. 1993: Gamma irradiation increases hsp-70 in chinese hamster ovary cells. Rad. Res. 135: 40-45 Go to original source...
  29. SONG L., WU L., NI D., CHANG Y., XU W. & XING K. 2006: The cDNA cloning and mRNA expression of heat shock protein 70 gene in the haemocytes of bay scallop (Argopecten irradians, Lamarck 1819) responding to bacteria challenge and naphthalin stress. Fish Shellfish Immunol. 21: 335-345 Go to original source...
  30. SORENSEN J.G. & LOESCHCKE V. 2001: Larval crowding in Drosophila melanogaster induces hsp70 expression, and leads to increased adult longevity and adult thermal stress resistance. J. Insect Physiol. 47: 1301-1307 Go to original source...
  31. TANAKA K., JAY G. & ISSELBACHER K.J. 1988: Expression of heat-shock and glucose-regulated genes: differential effects of glucose starvation and hypertonicity. Biochim. Biophys. Acta 950: 138-146 Go to original source...
  32. THOMPSON G.J., CROZIER Y.C. & CROZIER R.H. 2003: Isolation and characterization of a termite transferrin gene up-regulated on infection. Insect Mol. Biol. 12: 1-7 Go to original source...
  33. WAGNER M., HERMANNS I., BITTINGER F. & KIRKPATRICK C.J. 1999: Induction of stress proteins in human endothelial cells by heavy metal ions and heat shock. Am. J. Physiol. 277: L1026-L1033
  34. YOSHIGA T., HERNANDEZ V.P., FALLON A.M. & LAW J.H. 1997: Mosquito transferrin, an acute-phase protein that is upregulated upon infection. Proc. Natl. Acad. Sci. 94: 12337-12342 Go to original source...
  35. ZHANG G., SCHMIDTA O. & ASGARI S. 2006: A calreticulin-like protein from endoparasitoid venom fluid is involved in host hemocyte inactivation. Dev. Comp. Immunol. 30: 756-764 Go to original source...