Eur. J. Entomol. 105 (2): 205-210, 2008 | DOI: 10.14411/eje.2008.029

A chromosomal study on a Lebanese spittlebug Philaenus arslani (Hemiptera: Auchenorrhyncha: Aphrophoridae)

Anna MARYAŃSKA-NADACHOWSKA1, Valentina G. KUZNETSOVA2, Hani ABDUL-NOUR3
1 Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016 Kraków, Poland; e-mail: maryanska@isez.pan.krakow.pl
2 Department of Karyosystematics, Zoological Institute, Russian Academy of Sciences, 199034 St. Petersburg, Russia; e-mail: karyo@zin.ru
3 B.P. 90 549, Jdeidet al-Matn, 1202 2050 Lebanon, e-mail: hani-abd@inco.com.lb

The meadow spittlebug genus Philaenus (Auchenorrhyncha: Aphrophoridae) is known to display marked colour polymorphism. This study presents the results of a karyotype analysis of P. arslani from Lebanon using conventional chromosome staining, C-banding, fluorescent banding using base-specific fluorochromes (CMA3 and DAPI) and AgNOR-staining. This species has 2n = 18 + neo-XY, and differs from P. spumarius both in the number of chromosomes and sex chromosome system. During meiosis, the neo-XY bivalent is clearly heteromorphic being the largest in the complement. Furthermore, sex chromosomes show marked differences in C-banding pattern. The NOR-bearing chromosomes are the first and one of the middle-sized pairs of autosomes. NORs are G-C rich. Furthermore, some blocks of constitutive heterochromatin on the sex chromosomes are also G-C rich. All other C-bands are DAPI or DAPI/ CMA3 positive, thus containing A-T rich DNA. The significant difference in the karyotype of P. arslani and P. spumarius indicates chromosomal transformations during the evolution of the genus Philaenus.

Keywords: Hemiptera, Auchenorrhyncha, Aphrophoridae, Philaenus arslani, chromosomes, C-bands, DAPI, CMA3, NOR

Received: August 10, 2007; Accepted: November 27, 2007; Published: May 15, 2008

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