Eur. J. Entomol. 105 (1): 65-72, 2008 | 10.14411/eje.2008.009

Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera: Coreidae)

María José BRESSA1,3, María José FRANCO2, María Ayelén TOSCANI1, Alba GRACIELA PAPESCHI*,1,3
1 Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Int. Güiraldes 2160, C1428EGA, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Argentina; e-mail: alpape@ege.fcen.uba.ar
2 Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
3 Members of the Carrera del Investigador Científico (CONICET)

Heterochromatin is one of the most dynamic components in the genome of species. Previous studies on the heterochromatin content and distribution in Heteroptera (insects with holokinetic chromosomes) have shown that the species belonging to the family Coreidae are interesting model organisms since they show very diverse C bands patterns. In the present work, we analyzed the C-band pattern in individuals of Holhymenia rubiginosa from different populations collected in different years. This species has the diploid karyotype 2n = 27/28 = 24 + 2m + X0/XX (male/female). C-bands are terminally, subterminally or interstitially located on 10-17 chromosomes and a remarkable heterochromatin heteromorphism is observed in the meiotic bivalents: in the presence/absence of bands, in the size of bands and number of bands. A heteromorphism is also inferred in the number of ribosomal genes from the difference in the fluorescent in situ hybridization signals between NOR-homologues. Chiasmata are generally located opposite to conspicuous C-bands, but in some bivalents chiasmata are also observed in close proximity to C-bands. Considering the striking variation in heterochromatin content between individuals and populations it is suggested that heterochromatin should be selectively neutral in H. rubiginosa.

Keywords: Coreidae, holokinetic chromosomes, C-banding, fluorescent-banding, karyotype evolution, fluorescent in situ hybridization, rDNA

Received: June 6, 2007; Accepted: September 5, 2007; Published: February 15, 2008

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