Eur. J. Entomol. 105 (1): 45-52, 2008 | 10.14411/eje.2008.006

Experimental hybridization between X0 and XY chromosome races in the grasshopper Podisma sapporensis (Orthoptera: Acrididae). II. Cytological analysis of embryos and adults of F1 and F2 generations

Elżbieta WARCHAŁOWSKA-ŚLIWA1, Alexander G. BUGROV2, Yoshikazu SUGANO3, Anna MARYAŃSKA-NADACHOWSKA1, Shin-Ichi AKIMOTO3
1 Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016 Krakow, Poland; e-mail: warchalowska@isez.pan.krakow.pl; maryanska@isez.pan.krakow.pl
2 Novosibirsk State University, 630090 Novosibirsk and Institute for Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, 630091 Novosibirsk, Russia; e-mail: bugrov@fen.nsu.ru
3 Systematic Entomology, Department of Ecology and Systematics, Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589 Japan; e-mail: yoshikazu.sugano@nifty.com; akimoto@res.agr.hokudai.ac.jp

Experimental hybridization of X0 and XY chromosome races of the brachypterous grasshopper P. sapporensis did not reveal pre-zygotic reproductive isolation. However, a partial zygotic barrier was found between the X0-standard race from Shimokawa and XY-standard chromosome race from Akan. Approximately 40% of embryos from females crossed with males from other chromosome races developed parthenogenetically, the remaining embryos were normal heterozygotes. Adult F1 males and females from crosses of this type had properly developed testes and ovaries. Non-sister associations and other irregularities in meiosis were not observed in male meiosis. Crossing experiments demonstrated that hybrids between X0 and XY races occur to some extent. The absence of a hybrid zone between the X0 and XY chromosome races may be the result of selection against heterozygotes. Crosses between the XY-Tanno and X0-standard (Teine) subraces resulted in F1 and F2 generations in spite of the many chromosome differences between them such as a X-A translocation and fixed pericentric inversions in four pairs of autosomes. These results do not support the hypothesis that chromosomal differences play a key role in restricting gene flow between the X0 and XY races of P. sapporensis.

Keywords: Grasshoppers, Acrididae, Podisma sapporensis, chromosome races, experimental crossing, zygotic barrier

Received: April 26, 2007; Accepted: August 7, 2007; Published: February 15, 2008

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