Eur. J. Entomol. 105 (1): 35-39, 2008 | 10.14411/eje.2008.004

Silver-negative NORs in Pamphagus ortolaniae (Orthoptera: Pamphagidae)

Roberto VITTURI1, Antonella LANNINO1, Caterina MANSUETO1, Valentina MANSUETO1, Mariastella COLOMBA*,2
1 Dipartimento di Biologia Animale, UniversitL di Palermo, Via Archirafi 18, 90123 Palermo, Italy
2 Istituto di Ecologia e Biologia Ambientale, UniversitL di Urbino, Via Maggetti 22, 60129 Urbino (PU), Italy; e-mail: m.colomba@uniurb.it

The present paper reports some cytogenetic peculiarities observed in the Ag-NORs of Pamphagus ortolaniae chromosomes, the unusual behaviour of ribosomal sites after silver staining and the intense Ag-positive reaction of centromeric regions at spermatogonial metaphase and spermatocyte metaphase I and II. Moreover, a conclusive identification and localization of all the ribosomal clusters is provided by using heterologous rDNA FISH on spermatocyte chromosomes. 18S-28S rDNA mapped on a single chromosome pair and resulted multiclustered along the chromosomal body in three distinct serial regions, r1, r2 and r3. Surprisingly, these areas were scarcely (r1) or never (r2 and r3) detectable by silver impregnation. As in other Orthoptera and many groups of arthropods, FISH with the pentamer (TTAGG)n as the probe labelled the telomeres of all chromosomes.

Keywords: Ag-NOR, FISH, chromosome, rDNA, telomere, Pamphagus ortolaniae, Orthoptera, Pamphagidae

Received: March 22, 2007; Accepted: August 1, 2007; Published: February 15, 2008

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