Eur. J. Entomol. 99 (3): 267-276, 2002 | DOI: 10.14411/eje.2002.037

DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori

Balakathiresan N. SETHURAMAN, Thampiyath P. MOHANDAS, Shankar N. CHATTERJEE*
SeriBiotech Lab, Central Silk Board, Campus-Kodathi, Bangalore 560035, Karnataka, India

RFLP clones harbouring multi-copy DNA sequences were isolated from the Pst I sub-genomic library of the indigenous silkworm race, Nistari, and were used for DNA fingerprinting studies in 13 stocks of silkworm, Bombyx mori L. Six multilocus probes produced 180 RFLP markers that showed a high level (98%) of polymorphism and are highly useful in molecular mapping, genotype characterization and marker assisted selection (MAS). The dendrogram derived from UPGMA analysis clearly divides the 13 silkworm stocks into two major clusters: high- and low-yield stocks. Furthermore, adopting multiple regression analyses, the RFLP marker(s) associated with characters of economic importance were identified, a first of its kind for any species of insect of commercial importance. The results obtained create an opportunity of using germplasm stocks directly for isolating specific RFLP band(s) and use it for MAS in breeding programs.

Keywords: Bombyx mori, DNA fingerprinting, multilocus probes, Restriction Fragment Length Polymorphism, RFLP

Received: November 8, 2001; Accepted: May 8, 2002; Published: September 20, 2002

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References

  1. Barbosa-Neto J.F., Sorrells M. E. & Cisar G. 1996: Prediction of heterosis in wheat using coefficient of parentage and RFLP-based estimates of genetic relationship. Genome 39: 1142-1149 Go to original source...
  2. Bark O.H. & Havey M.J. 1995: Similarities and relationships among populations of the bulb onions as estimated by nuclear RFLPs. Theor. Appl. Genet. 90: 407-414 Go to original source...
  3. Blanchetot A. 1991: A Musca domestica satellite sequence detects individual polymorphic regions in insect genome. Nucleic Acids Res. 19: 929-932 Go to original source...
  4. Botstein D., White R.L., Skolnick M. & Davis R.W. 1980: Construction of a genetic map in man using restriction fragment length polymorphisms. Am. J. Human. Genet. 32: 314-331
  5. Chatterjee S.N. & Datta R.K. 1992: Hierarchical clustering of 54 races and strains of the mulberry silkworm, Bombyx mori L: Significance of biochemical parameters. Theor. Appl. Genet. 85: 394-402 Go to original source...
  6. Chatterjee S.N. 1993: Silkworm breeding in India. Sericologia 33: 427-447
  7. Chatterjee S.N., Rao C.G.P., Chatterjee G. K., Ashwath S. K. & Patnaik A.K. 1993: Correlation between yield and biochemical parameters in the mulberry silkworm, Bombyx mori L. Theor. Appl. Genet. 87: 385-391 Go to original source...
  8. Currie R. 1996: Changing pattern in international demand for silk and silk goods. In Global silk scenario-2001. Proc. Intl. Conference in Sericulture, 1994. Oxford & IBH Publ., Calcutta, India, pp. 37-43
  9. Dos Santos J.B., Nienhuis J., Skroch P., Tivang J. &. Slocum M. K. 1994: Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes. Theor. Appl. Genet. 87: 909-915 Go to original source...
  10. Felsenstein J. 1989: PHYLIP-phylogeny inference package (Version 3.2) Cladistics 5: 164-166
  11. Ghosh C.C. 1949: Silk Production and Weaving in India. CSIR Monograph, India, pp. 13-17
  12. Goldsmith M.R. 1991: Silkworm breeding for the "90"s: New molecular and genetic tools to meet the challenge of the tropics. Sericologia 31: 145-155
  13. Hahn V., Blankenhorn K., Schwall M. & Melchinger A.E. 1995: Relationships among early European maize inbreds: III. Genetic diversity revealed with RAPD markers and comparison with RFLP and pedigree data. Maydica 40: 299-310
  14. Jeffreys A.J., Wilson V. & Thein S.L. 1985: Individual specific "fingerprints" of human DNA. Nature 316: 76-79 Go to original source...
  15. Jeffreys A.J., Royle N.J., Patel I., Armour J.A.L., MacLeod A., Collic A., Gray I.C., Neumann R., Gibbs M., Crosier M., Hill M., Singer E. & Monckton D. 1991: Principles and Recent Advances in Human Fingerprinting: Approaches and Applications. Birkhauser Verlag, Basel, pp. 1-19
  16. Jung C., Pillen K., Frese L., Fahr S. & Melchinger A.E. 1993: Phylogenetic relationships between cultivated and wild species of the genus Beta revealed by DNA fingerprining. Theor. Appl. Genet. 86: 449-457 Go to original source...
  17. Landry B.S., Kesseli R., Leung H. & Michaelmore R.W. 1987: Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.). Theor. Appl. Genet. 74: 646-653 Go to original source...
  18. Lu J., Knox M. R., Ambrose M.J., Brown J.K.M. & Ellis T.H.N. 1996: Comparative analysis of genetic diversity in pea assessed by RFLP-and PCR-based methods. Theor. Appl. Genet. 93: 1103-1111 Go to original source...
  19. Lynch M. 1999: Estimating genetic correlations in natural populations. Genet. Res. 74: 255-264 Go to original source...
  20. Messmer M.M., Melchinger A.E., Hermann R.G. & Boppenmeir J. 1993: Relationship among early European maize inbreds II. Comparison of pedigree and RFLP data. Crop. Sci. 33: 944-950 Go to original source...
  21. Michaelmore R.W., Paran I. & Kesseli R.V. 1991: Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828-9832 Go to original source...
  22. Miller J.C. & Tanksley S.D. 1990: RFLP analysis of phylogenetic relationships and genetic variation in genus Lycopersicon. Theor. Appl. Genet. 80: 437-448 Go to original source...
  23. Mitra A., Yadav B.R., Ganai N.A. & Balakrishnan C.R. 1999: Molecular markers and their applications in livestock improvement. Curr. Sci. 77: 1045-1053
  24. Mukherjee N.G. 1912: Handbook of Sericulture. Govt. Book Depot, Calcutta, India. Nagaraja G.M. & Nagaraju J. 1995: Genome fingerprinting of the silkworm, Bombyx mori, using random arbitrary primers. Electrophoresis 16: 1633-1638 Go to original source...
  25. Nagaraju J., Sharma A., Sethuraman B.N., Rao G.V. & Singh L. 1995: DNA fingerprinting in silkworm Bombyx mori using banded krait minor satellite DNA - derived probe. Electrophoresis 16: 1639-1642 Go to original source...
  26. Nei M. & Li W.H. 1979: Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. U.S.A 76: 5269-5273 Go to original source...
  27. Neuhusen S.L. 1992: Evaluation of restriction fragment length polymorphism in Cucumis melo. Theor. Appl. Genet. 83: 379-384 Go to original source...
  28. Noli E., Salvi S. & Tuberosa R. 1997: Comparative analysis of genetic relationships in barley based on RFLP and RAPD markers. Genome 40: 607-616 Go to original source...
  29. O'Brien S.J. 1993: Genetic Maps 6th ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 4.1-4.52
  30. Paterson A.H., Lin Y.R., Li Z., Schertz K.F. & Dobbley J.F. 1995: Convergent domestication of cereal crops by independent mutation at corresponding genetic loci. Science 269: 1714-1718 Go to original source...
  31. Paull J.G., Chalmers K.J., Karakousis A., Kretsehmer J.M., Manning S. & Langridge P. 1998: Genetic diversity in Australian wheat varieties and breeding material based on RFLP data. Theor. Appl. Genet. 96: 435-446 Go to original source...
  32. Pejic I., Marsan P.A., Morgante M., Kozumplik V., Castiglioni P., Taramino G. & Motto M. 1998: Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs. Theor. Appl. Genet. 97: 1248-1255 Go to original source...
  33. Powel W., Morgante M., Andre C., Hanafey M., Vogel J., Tingey S. & Rafalski A. 1996: The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed. 2: 225-238 Go to original source...
  34. Reddy K.D., Abraham E.G. & Nagaraju J. 1999a: Microsatellites in the silkworm, Bombyx mori: Abundance, polymorphism, and strain characterization. Genome 42: 1057-1065 Go to original source...
  35. Reddy K.D., Abraham E.G. & Nagaraju J. 1999b: Genetic characterisation of silkworm, Bombyx mori by simple sequence repeat (SSR)-anchored PCR. Heredity 83: 681-687 Go to original source...
  36. Salimath S.S., de Oliveira A.C., Godwin I.D. & Bennetzen J.L. 1995: Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers. Genome 38: 757-763 Go to original source...
  37. Sambrook J., Fritsch E.F. & Maniatis T. 1989: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold spring Harbor, N.Y. Shi J., Heckel D.G. & Goldsmith M.R. 1995: A genetic linkage map for the domesticated silkworm, Bombyx mori, based on Restriction Length Polymorphisms. Genet. Res., Camb. 66: 109-126
  38. Smith O.S., Smith J.S.C., Bowen S.L., Tenborg R.A. & Wall S.J. 1990: Similarities among a group of elite maize inbreds as measured by pedigree, F1 heterosis, and RFLPs. Theor. Appl. Genet. 80: 833-840 Go to original source...
  39. Song K., Osborne T.C. & Wiiliams P.H. 1990: Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 3. Genome relationships in Brassica and related genera and the origin of B. oleracea and B. rapa (syn. Campestris). Theor. Appl. Genet. 79: 497-505 Go to original source...
  40. Stomberg L.D., Dudley J.W. & Rufener G.K. 1994: Comparing conventional early generation selection with molecular marker assisted selection in Maize. Crop Sci. 34: 1221-1225 Go to original source...
  41. Sulaiman I.M., Ehtesham N.Z. & Hasnain S.E. 1995: A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy. Gene 156: 223-227 Go to original source...
  42. Tanksley S.D., Ganal M.W., Prince J.P., Dvicente M.C. & Bonierbale M.N. 1992: High diversity molecular linkage maps of tomato and potato genomes. Genetics 132: 1141-1160
  43. Tanksley S.D., Young N.D., Paterson A.H. & Bonierbale M.W. 1989: RFLP mapping in plant breeding: New tools for an old science. Biotechnol. 7: 257-264 Go to original source...
  44. Thangavelu K., Mukherjee P., Sinha R.K., Mahadevamurthy T.S., Sunitha M., Sahni N.K., Kumaresan P., Rajarajan P.A., Mohan B. & Sekar S. 1997: Catalogue on Silkworm (Bombyx mori L.) Germplasm Vol. 1. Silkworm and Mulberry Germplasm Station, Hosur, Tamil Nadu, India, pp. 79-138
  45. Tivang J.G., Nienhuis J. & Smith O. 1994: Estimation of sampling variance of molecular marker data using the boot strap procedure. Theor. Appl. Genet. 89: 259-264 Go to original source...
  46. Virk P.S., Ford-Lloyd B.V., Jackson M.T., Pooni H.S., Clemeno T.P. & Newbury H.J. 1996: Predicting quantitative variation within rice germplasm using molecular markers. Heredity 76: 296-304 Go to original source...
  47. Yonash N., Heller E.D., Hillel J. & Cahanar A. 2000: Detection of markers associated with antibody response in meat-type chickens: Haplotype/genotype, single-band, and multiband analyses of RFLP in the major histocompatibility complex. J. Heredity 91: 24-30 Go to original source...